ppsA [2019-04-16 12:27:48]

plipastatin synthetase

locus: BSU18340

pI: 5.22

mw: 288.86 kDa

protein length: 2561 aa

gene length: 7686 bp

function: production of the antibacterial compound plipastatin

product: plipastatin synthetase

essential: no

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.6|Miscellaneous metabolic pathways] → [category|SW 2.6.6.1|Biosynthesis of antibacterial compounds]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.15|Biosynthesis of antibacterial compounds]

Gene

Coordinates: 1,990,272 → 1,997,957

The protein

Protein family

• ATP-dependent AMP-binding enzyme family (according to Swiss-Prot)

Structure

• [PDB|2VSQ] (termination module of [protein|1D5F227860A321506A1AB4BAE63CAD3A66E43BFC|SrfAC], 30% identity) [pubmed|18583577]

Expression and Regulation

Operons

• genes: [gene|E3D7EF8F165CAC2682B0D620081C5C6E555E43C4|ppsA]-[gene|E7A2F93B33B1899313893D362D92405232BC9097|ppsB]-[gene|D11CE89E2AC2ADE3FEFEC261E295EC5906CE6407|ppsC]-[gene|E73D9F1A8F54DAD4B2056CABBE580D7AD36CB042|ppsD]-[gene|C1C051209943E02369356075EAA733297E9478CB|ppsE]
  description: [Pubmed|20817675]

  regulatory mechanism

  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|20817675], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]

Biological materials

Mutant

• BKE18340 (Δ[gene|E3D7EF8F165CAC2682B0D620081C5C6E555E43C4|ppsA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE18340 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACGGATTCCCTCCAGTTC, downstream forward: _UP4_TAACTTTTGAAAAGGTGTGT
• BKK18340 (Δ[gene|E3D7EF8F165CAC2682B0D620081C5C6E555E43C4|ppsA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK18340 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACGGATTCCCTCCAGTTC, downstream forward: _UP4_TAACTTTTGAAAAGGTGTGT

References

• 10471562,9387222,20817675,18583577